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bio‐rad iq5 real‐time pcr machine  (Bio-Rad)


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    Bio-Rad bio‐rad iq5 real‐time pcr machine
    Bio‐Rad Iq5 Real‐Time Pcr Machine, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bio+rad+iq5+machine/CFX96+and+CFX384+Real-Time+PCR+Detection+Systems+Firmware+Update/pmc09398900-270-10-9
    Average 90 stars, based on 1 article reviews
    bio‐rad iq5 real‐time pcr machine - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: The Arabidopsis BET Bromodomain Factor GTE4 Is Involved in Maintenance of the Mitotic Cell Cycle during Plant Development
    Article Snippet: Invitrogen SuperScriptII was used to reverse transcribe the RNA following the manufacturer's instructions. .. Real-time PCR was performed with a Bio-Rad IQ5 machine using IQ Bio-Rad SYBR Green Supermix (catalog no. 170-8882) and the primers listed below. ..

    Article Title: FLOWERING LOCUS T mRNA is synthesized in specialized companion cells in Arabidopsis and Maryland Mammoth tobacco leaf veins
    Article Snippet: DNase treatment was performed using on-column DNase (Sigma-Aldrich) in accordance with the manufacture’s recommendation. cDNA was synthesized using 2 μg of total RNA, 100 pmol Oligo dT, 10 nmol dNTP, 20 U of RiboLock RNase inhibitor (Thermo Fisher Scientific), and 200 U of RevertAid reverse transcriptase (Thermo Fisher Scientific) in a 20-μL reaction. .. Quantitative PCR was performed in 96-well plates on a Bio-Rad iQ5 machine using iTaq universal SYBR Green Supermix (Bio-Rad) in a 12-μL reaction. ..

    SYBR Green Assay:

    Article Title: The Arabidopsis BET Bromodomain Factor GTE4 Is Involved in Maintenance of the Mitotic Cell Cycle during Plant Development
    Article Snippet: Invitrogen SuperScriptII was used to reverse transcribe the RNA following the manufacturer's instructions. .. Real-time PCR was performed with a Bio-Rad IQ5 machine using IQ Bio-Rad SYBR Green Supermix (catalog no. 170-8882) and the primers listed below. ..

    Article Title: FLOWERING LOCUS T mRNA is synthesized in specialized companion cells in Arabidopsis and Maryland Mammoth tobacco leaf veins
    Article Snippet: DNase treatment was performed using on-column DNase (Sigma-Aldrich) in accordance with the manufacture’s recommendation. cDNA was synthesized using 2 μg of total RNA, 100 pmol Oligo dT, 10 nmol dNTP, 20 U of RiboLock RNase inhibitor (Thermo Fisher Scientific), and 200 U of RevertAid reverse transcriptase (Thermo Fisher Scientific) in a 20-μL reaction. .. Quantitative PCR was performed in 96-well plates on a Bio-Rad iQ5 machine using iTaq universal SYBR Green Supermix (Bio-Rad) in a 12-μL reaction. ..



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    Image Search Results


    Bromodomain and extraterminal (BET) inhibition reduced hepatocyte proliferation in the zebrafish acetaminophen (APAP)-induced injury model. A: Scheme illustrating the periods of APAP and JQ1 treatments and analysis stages (arrows). B: Confocal images showing the hepatic expression of fabp10a:CFP (gray, hepatocytes), Tp1:H2B-mCherry (red, biliary epithelial cells), and fabp10a:mAGFP-gmnn (green, proliferating hepatocytes). C: Quantification of the number of mAGFP-gmnn+ cells, as shown in B. D–H: Real-time quantitative PCR data showing the relative expression levels of ccnd1, pcna, myca, brd4, and axin2 among uninjured control livers at 5 days after fertilization (dpf) and dimethyl sulfoxide (DMSO)- and JQ1-treated regenerating livers at 12 hours of recovery (R12h). Data are expressed as means ± SEM. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗∗P < 0.0001 (one-way analysis of variance). Scale bar = 100 μm. CFP, cyan fluorescent protein.

    Journal: The American Journal of Pathology

    Article Title: Bromodomain and Extraterminal (BET) Proteins Regulate Hepatocyte Proliferation in Hepatocyte-Driven Liver Regeneration

    doi: 10.1016/j.ajpath.2018.02.006

    Figure Lengend Snippet: Bromodomain and extraterminal (BET) inhibition reduced hepatocyte proliferation in the zebrafish acetaminophen (APAP)-induced injury model. A: Scheme illustrating the periods of APAP and JQ1 treatments and analysis stages (arrows). B: Confocal images showing the hepatic expression of fabp10a:CFP (gray, hepatocytes), Tp1:H2B-mCherry (red, biliary epithelial cells), and fabp10a:mAGFP-gmnn (green, proliferating hepatocytes). C: Quantification of the number of mAGFP-gmnn+ cells, as shown in B. D–H: Real-time quantitative PCR data showing the relative expression levels of ccnd1, pcna, myca, brd4, and axin2 among uninjured control livers at 5 days after fertilization (dpf) and dimethyl sulfoxide (DMSO)- and JQ1-treated regenerating livers at 12 hours of recovery (R12h). Data are expressed as means ± SEM. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗∗P < 0.0001 (one-way analysis of variance). Scale bar = 100 μm. CFP, cyan fluorescent protein.

    Article Snippet: Real-time quantitative PCR (qPCR) was performed as previously described, 33 using the Bio-Rad iQ5 qPCR machine with the iQ SYBR Green Supermix (Bio-Rad).

    Techniques: Inhibition, Expressing, Real-time Polymerase Chain Reaction, Control